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Single-cell RNA Analysis References

Detailed contracts are split by decision point so the agent only loads the material needed for the current stage.

Files

File Use it for
input-contract.md Versioned configuration, sample-sheet and h5ad requirements, count validation, identifiers, reference consistency, contrast and confounding checks.
scientific-workflow.md Per-sample QC, Scrublet, preserved representations, optional Harmony, resolution sweep, clustering and descriptive markers.
annotation-contract.md Marker-panel evidence, conflicts, reference compatibility, Unknown, and user-confirmed mappings.
statistics-contract.md Donor-aware pseudobulk DE, pairing, explicit design formulas, replication gates and Milo DA.
output-contract.md Checkpoints, resume invalidation, structured statuses, report, manifest, checksums and Artifact registration.
README_zh.md Chinese directory guide with the same structure as this file.

These documents describe scientific and execution contracts, not biological ground truth or a substitute for study-specific review.