Echo-MS Detection of Molecules from an Enzymatic Reaction (Cell Free)

URL: https://cloud.ginkgo.bio/protocols/echo-ms-cfps-detection Status: Beta Price: $44/sample Turnaround: up to 13 days

Overview

Fastest path from a protein sequence to a functional, quantitative readout on enzyme activity. Using a proprietary reconstituted E. coli transcription-translation (CFPS) system, Ginkgo expresses your enzyme of interest in 4-16 hours. A substrate or product is then added directly to the well and substrate conversion is measured by acoustic ejection mass spectrometry (Echo-MS), delivering a go/no-go signal without protein purification. Enzyme expression and Echo-MS are performed using the baseline Cell Free Protein Synthesis Master Mix.

To run this protocol, the relevant analyte/method must first be onboarded (see echo-ms-method-onboarding.md).

Input

• DNA Input: Enzyme construct(s)
• Reaction substrate/product and reaction conditions (provided with the order)

Output

• Method summary
• Peak table (substrate depletion and/or product formation)

Automated Workflow

Phase 1 - CFPS Expression

1. Express enzyme in CFPS master mix (4-16 h)

Phase 2 - Enzymatic Reaction

1. Dispense molecule, buffers, reagents (Agilent Bravo 96)
2. Incubate under reaction conditions (Inheco)

Phase 3 - Echo-MS Detection

1. Acoustic ejection mass spectrometry readout of substrate/product

Ordering

• Number of Samples: configurable ($44/sample)
• File Upload: CSV, Excel, FASTA, TXT, PDF, ZIP
• Additional Details: free-text field for special requirements

Use Cases

• Go/no-go enzyme activity screening without purification
• Detecting substrate depletion / product formation for biocatalysis
• High-throughput functional triage of enzyme variants