skills/bioskills/bio-genome-engineering-grna-design/scripts/grna_design.py

• gc_fraction · function · L23-L24 — def gc_fraction(spacer)
• find_guides · function · L27-L46 — def find_guides(sequence, guide_length=GUIDE_LEN)
• passes_u6_filters · function · L49-L51 — def passes_u6_filters(spacer)
• prepend_u6_g · function · L54-L56 — def prepend_u6_g(spacer)
• out_of_frame_score · function · L59-L89 — def out_of_frame_score(local_seq, cut)
• design_guides · function · L92-L110 — def design_guides(sequence, delivery='u6', n_guides=5)